Wide-area, UV-induced, high-brightness photodiodes known as Si APDs are ideal for a variety of applications that require low-light-level, broadband components. These products are commonly used in flat-pack packages. The low noise, high gain, and low-capacitance of these products make them ideal for direct detection and easy coupling to crystal structures. Below we shall break down on how the fluorescence detectors work and their application in the industry.
How The Fluorescence Detector Works
A type of luminescence known as fluorescence occurs when a molecule absorbs light in the form of light. It then releases a photon, which causes the molecule to become an excited electronic state. It then decays to the ground in an unexcited state. The photon emitted by the fluorescent molecule is always lower in energy than the absorbed light. The wavelength of light is proportional to the energy. Since the emitted radiation has a longer wavelength than the absorbed light, the emitted photon is always lower in energy than the absorbed light.
The design of a fluorescence detector cell allows it to detect the emission of photons at a specific wavelength. It can also be equipped with a parabolic reflector to maximize the number of photons that it can collect. Usually, a fluorescence detector excites a fluorophore with a specific wavelength, and then monitors its emission at a different wavelength.
The light from the excitation is then removed by either a second filter or a monochromator, which allows the absorbed light to strike the transducer. A small number of compounds can exhibit native fluorescence. The more double bonds present in a molecule, the greater its fluorescence intensity. Because of this, selective detectors are only used for detecting a limited number of fluorescent molecules.
The choice of the excitation and emission wavelengths of a fluorescent detector can also improve its selectivity. Ideally, interfering components should not be detected if they either don’t absorb at the excitation wavelength or don’t emit at the emission wavelength. In addition, fluorescence is very useful for detecting fluorescent molecules due to its noise-free properties. A series of fluorescent detectors can be used to monitor the signals from both the emission and excitation wavelengths. This allows them to improve their sensitivity and selectivity.
Applications Of the Fluorescence Detector
A fluorescence detection instrument is commonly used for analysis when the sensitivity and selectivity of the analyte are required. It can also be used to derivatize the analyte to produce fluorescence. Although high-sensitivity analysis using mass spectrometry is becoming more prevalent, fluorescence detection by HPLC has been regarded as the official method for this procedure. This is mainly because when the analyte has very little or no UV absorbance, it can be derivate to produce fluorescence.
Mass spectrometry is commonly used for this type of analysis, though HPLC is also regarded as the official method for fluorescence detection. This type of detection is usually performed when the analyte has no UV exposure and can be derivatized to produce fluorescence. Mass spectrometry is commonly used for this type of analysis, though HPLC is also regarded as the official method for fluorescence detection.